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Kristen J. Navara

We believe in openly sharing our lab protocols with anyone who might need them. Feel free to use them and if you would like to collaborate with us, contact Dr. Navara!

Genomic DNA Salt Extraction (can be used for tissue and germinal discs)

PCR sexing for chicken, quail, and zebra finches

DNA extraction and Molecular Sexing Protocols

Bactericidal Assay for Plasma

Protocols for Testing Immune Function

Phytohemagglutinin (PHA) skin swelling test for passerines and chickens

Lipopolysaccharide (LPS) febrile challenge

Carbon clearance in vitro phagocytosis assay

Peritoneal macrophage extraction and challenge with LPS

List of immune challenges and what they measure

Diethyl ether extraction of hormones from plasma

Protocols for Hormone Measurement

Extraction and column chromatography for isolation of hormones from yolk and whole body tissue

Extraction of fecal corticosterone

Extraction of hair cortisol (used so far for bat and cotton rat hair)

List of ELISAs and RIAs that we currently use in the lab (with catalog numbers)

In-house radioimmunoassay protocol

Sperm hole penetration assay

Sperm mobility assay

Nigrosin/Eosin stain recipe

Protocols for Testing Sperm Quality

Sperm viability assay

Staining for Yolk Rings

Additional Protocols

Need a way to isolate a frozen yolk without breaking or thawing it?

Check this out! (egg must be covered in dry ice first!)

Watch how dipping a yolk in water makes the germinal disc stand out!

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